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KMID : 1134820110400030385
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Journal of the Korean Society of Food Science and Nutrition
2011 Volume.40 No. 3 p.385 ~ p.392
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Co-Culture Model Using THP-1 Cell and HUVEC on AGEs-Induced Expression of Cytokines and RAGE
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Lee Kwang-Won
Lee Hyun-Sun
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Abstract
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Although monoculture methods have been remarkably useful due to their simplicity, they have serious limitation because of the different types of cells communication with each other in many physiological situations. We demonstrated levels of markers of endothelial dysfunction such as tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) as well as stimulation of receptor of advanced glycation endproducts (AGEs) on monoand co-culture system such as only monocyte (THP-1) cultivation system, only endothelial cell (HUVEC) cultivation system, and co-cultivation system of THP-1 and HUVEC. The mRNA levels of TNF-α and IL-1β on HUVEC increased by the co-culture with monocyte after 4 hr at 100 ㎍/mL glyceraldehyde-AGE. The secreted protein contents into medium of TNF-α and IL-1β increased after 8 hr approximately 2～2.5 times compared to mono-cultivation. In contrast, the mRNA level of receptor of AGE (RAGE) was relatively insensitive on the co-culture system. The mediators by which monocytes activate endothelial cell have not been fully elucidated. In this study we confirmed production of soluble cytokines such as TNF-α and IL-1β by monocytes. Use of monocyte conditioned medium, which contains both cytokines, can activate endothelial cell.
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KEYWORD
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advanced glycation endproducts (AGEs), endothelial dysfunction, co-culture, endothelial cell, monocyte
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